Can anyone help me to separate my phenolic compound in TLC with any new solvent system ?

My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.

22 January 2021 4,693 7 View

Which journal is suitable to send manuscript related to survey on ethnobotanical usage of medicinal plants with no publication charges?

survey is related to diversity of medicinal plants used for treating different diseases

13 October 2019 9,985 2 View

No title

It seems that parenting style is considered as a constant pattern of parental behavior adopted by parents while dealing with their children. Can same parents adopt different parenting styles to...

11 February 2019 5,048 3 View

Can anyone help me out in interpretation of a powder XRD diffractogram?

I did the xrd for bentonite. I compared the peaks with the standard database but the peaks didn't match with montmorillonite. How is the analysis done?

04 August 2017 8,513 7 View

Difference in heat transfer properties of natural and synthetic graphite

explain the Difference in heat transfer properties of natural and synthetic graphite

22 June 2017 4,647 1 View

How I can coat porous metal, oxides nanowires or nanoparticles in the channels of 0.3 micrometer width and 0.5 micrometer depth????

suggest method of coating...

03 May 2017 4,382 1 View

I want to know how to search for promoter of any genes? Specifically I want the full promoter sequence of Aspergillus Nidulans alcA promoter?

I am planning to make an overexpression plasmid with alcA promoter and trpC terminater. Can anyone provide me the sequence of alcA promoter or even a plasmid map of such expression vector that you...

13 February 2017 857 3 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

10 March 2015 1,392 4 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

10 April 2014 7,464 8 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

18 March 2014 423 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View