Eliciting target response from children, without much syntactic complexity?

We are developing a test for ad-hoc (ad-hoc) and scalar implicatures (SI) and are showing 3 images (of similar nature) to the participants: image, image with 1 item, image with 2 items. E.g....

17 January 2021 9,317 2 View

How do you culture GSCs cells - problem?

Dear all, I culture GSCs derived from patients. Few weeks ago I started having problem with them. They dont want to grow and make spheres. They are single cells I culture them in Neurobasal A...

16 November 2020 9,801 3 View

Which LPS is best to obtain high level of TNF-alpha in RAW264.7 cells?

I'm struggling a bit with the TNFalpha assay using RAW264.7 cells: 1. LPS is leading to only an 80% increase in TNFalpha mRNA levels, which seems low. 2.Increasing the dose of LPS leads to a...

09 October 2015 8,748 9 View

Which LPS is best to obtain high level of TNF-alpha in RAW264.7 cells?

I'm struggling a bit with the TNFalpha assay using RAW264.7 cells: 1. LPS is leading to only an 80% increase in TNFalpha mRNA levels, which seems low. 2.Increasing the dose of LPS leads to a...

08 October 2015 9,523 8 View

Can I stain membrane with Ponceau S after staining with both antibodies?

Hello, I wonder if I can stain nitrocellulose membrane with Ponceau S but not directly after transfer as it's usual do, but after staining with both antibodies (primary and secondary) and...

09 July 2015 7,061 6 View

Can I stain membrane with Ponceau S after staining with both antibodies?

Hello, I wonder if I can stain nitrocellulose membrane with Ponceau S but not directly after transfer as it's usual do, but after staining with both antibodies (primary and secondary) and...

06 July 2015 7,555 7 View

Can anyone give me technical advice on Western Blot analysis of KRAS protein?

07 January 2015 1,934 1 View

Which KRAS antibody would you recommend for Western Blot analysis?

Hello, I would like to ask for help everyone who has some experience with KRAS protein detection by Western Blot Analysis. Could you recommend me any good quality and good working KRAS antibody...

31 December 2014 4,411 4 View

CfP: EAA 2021 Session: Between Bones and Beliefs: Human-Bird Relations in Central and Northern Europe in the 1st Millennium AD

Human-avian relationships developed in many ways throughout the Iron Age, particularly in the 1st millennium AD in Central and Northern Europe. These airborne animals foraged and scavenged...

01 January 1970 296 1 View

Eliciting target response from children, without much syntactic complexity

We are developing a test for ad-hoc (ad-hoc) and scalar implicatures (SI) and are showing 3 images (of similar nature) to the participants: image, image with 1 item, image with 2 items. Eg....

01 January 1970 5,251 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View