Klaudia Kloc-Muniak

4 Questions 3 Answers 0 Followers

Questions related from Klaudia Kloc-Muniak

Which LPS is best to obtain high level of TNF-alpha in RAW264.7 cells?

I'm struggling a bit with the TNFalpha assay using RAW264.7 cells: 1. LPS is leading to only an 80% increase in TNFalpha mRNA levels, which seems low. 2.Increasing the dose of LPS leads to a...

09 October 2015 9,620 8 View

Can I stain membrane with Ponceau S after staining with both antibodies?

Hello, I wonder if I can stain nitrocellulose membrane with Ponceau S but not directly after transfer as it's usual do, but after staining with both antibodies (primary and secondary) and...

10 July 2015 7,191 6 View

Can anyone give me technical advice on Western Blot analysis of KRAS protein?

Hello, I would like to measure the level of KRAS protein by Western Blot analysis. Does anyone have experience with KRAS protein detection by Western Blot. Can you give me soem technical advice,...

08 January 2015 2,041 1 View

Which KRAS antibody would you recommend for Western Blot analysis?

Hello, I would like to ask for help everyone who has some experience with KRAS protein detection by Western Blot Analysis. Could you recommend me any good quality and good working KRAS antibody...

08 January 2015 8,720 3 View