I read CellSearch (Veridex) from the articles but it is very expensive sytem.
It also is based on the normal epithelial cell surface marker, EpCAM. Cancer cells are well known to change their molecular makeup, including through epithelial to mesenchymal transition, loosing EpCAM expression in the process. Thus they, the CTCs, become "invisible" to CellSearch. Worse: it is the most malignant cells that become invisible!
the EpCAM-enriched method is the gold standard (Veridex is FDA approved), as rightly wrote Christer Ericsson, the EMT process is likely to downregulates the EpCAM expression making difficult the enrichment of all CTCs.
There are methods based on physical characteristics as the classic Ficoll, or the newest RosetteSep.
Since there is not a perfect enrichment method, as far as I am concerned, your choice should be balanced according to what you want to do after CTCs enrichment.
We used a combination of cytokeratin and EpCam-MicroBeads (Miltenyi Biotech GmbH) to isolate CTCs from metastatic breast cancer patients: http://www.biomedcentral.com/1471-2407/12/206/abstract
NSCLC is Epcam positive and immuno-magnetic isolation using Epcam antibodies can work.
SCLC is mostly Epcam negative and isolation on basis of Epcam does not work. Filtration might be a good alternative.
We have developed a new filtration method for the isolation of CTC.
Please take a look at our website. A demonstration of the method can be viewed here : http://vycap.com/Whole%20blood%20filtration.wmv
the best method is Cell search system that have FDA approved
but its need some special tools !!!
I offer you to use MACS column for isolation .you can do this manually with high yield .
this method base on immunomagnetic separation that the best marker that I can suggest you as Mr. Christer Ericsson mention EpCAM is the best marker for isolation CTCs with epithelial origin such breast cancer,CRC,lung,...
but may you will use some of your CTCs related to EMT process and EpCAM WILL BE DECREASE ON CTCS!
please read the comparison publication.
Article Comparing Sequential Steps For Detection Of Circulating Tumo...
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