Which is the most interesting research study of Latinxs during pandemic??

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Has anyone use Emx1Cre adult mice to label pyramidal neurons?

I want to inject a Cre dependent virus to be expressed in pyramidal neurons, and I have Emx1Cre adult mice. I know Emx1 is a developmental gene, but found a paper showing expression of Emx1 also...

28 March 2024 1,666 0 View

Genotyping plants for overexpression insertion?

I have plants (L. japonicus) that have been transformed with an overexpression plasmid. How can I know that these plants are homozygous for the insertion?

24 March 2024 8,533 2 View

Are there any guidelines for using CHAT GPT in your research paper title?

I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...

12 February 2024 7,730 1 View

OMVs labeling for uptake assay?

Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...

03 October 2023 2,929 3 View

Problem with F-AAS Zinc quality controls standards?

Hello, in our lab we use F-AAS for determination of Mg in urine and Li, Zn in serum. Except Zinc QC 2 everything works fine. Is it possible there is problem with zinc controls? I am waiting for...

04 September 2023 4,169 1 View

Does anyone has a CamKII primary antibody to recommend for immunostaining in slices?

Hi! Im experiencing problems with CamKII immunostaining for free floating mouse PFC and Hp slices. I ve tried different blocking agents ( NDS, NGS, BSA in different percentages), different mouse...

30 July 2023 7,341 0 View

How to CRISPR-KO and CRISPR-HDR of p53 in fibroblasts?

I performed CRISPR-KO of p53 in fibroblasts. I've got cells that lost the activity of WTp53 (several target genes tested on qPCR) but on western blot, I see still quite a high level of a protein...

15 January 2023 8,080 0 View

ImageJ or QuPath?

How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?

03 July 2022 6,002 3 View

ReproSil NH2 column for sugars analysis - mobile phase?

I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...

08 June 2022 2,467 0 View

What polymeric material have the most similar properties as nylon 6.6 , and it is not from the polyamides family?

What polymeric material has the most similar properties as nylon 6.6 in terms of the processing and mechanical properties, and it is not from the polyamides family?

05 August 2021 6,193 8 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

01 July 2024 889 3 View

What factors contribute to educational apathy in South Dakota high school students post-pandemic?

The main variable of interest in the study is educational apathy with contributing factors that can include: mental health, socioeconomic status, school environment, academic performance, remote...

15 June 2024 1,028 2 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Is distance education effective as Onsite education?

Since the experience of COVID-19 in the world, educational bodies, institute of higher learning has adopted distance education. Even though the use has existed ever before the COVID-19 issue....

22 May 2024 7,430 3 View

Where to perform invitro transcription and capping?

I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?

14 May 2024 186 3 View

Is COVID vaccine harmful to cancer or beneficial?

Frankly speaking thrice time affected by COVID although covaxin taken but no harm to cancer patients.it depending upon metastatic position at that time of COVID affected person s . I was patient...

02 May 2024 1,818 3 View

How has language, particularly around the unquestionable authority of 'science' been used to uphold the dominance of hyper-elites?

How has language, particularly around the unquestionable authority of 'science' (vs. other domains of inquiry that apply rigorous method but are not generally considered 'science') been used to...

20 April 2024 1,146 4 View

Sequences of mRNA vaccines?

For my project I am looking for the mRNA sequences of mRNA vaccines that are currently undergoing clinical trails or that are already in use. I know that the sequences of two Covid-19 vaccines are...

16 April 2024 639 1 View