If we use an oil-immersion objective in dry conditions (air), will the maximum collection angle of the objective change?

What if we use an immersion oil objective in the air (medium between the objective and the sample)? Will the effective NA change? What about the maximum collection angle?

22 January 2020 7,842 1 View

Is 6-311G APFD-DFT++ a right choice for carrying out an opt+freq calculation of cyclopentyl methyl ether and cyclopentanol?

I want to optimize Cyclopentanol and cyclopentylmethyl ether using Gaussian.

12 January 2019 8,515 4 View

How to find structurally similar protein?

I have a protein obtained through ab initio modeling. I would like to know whether this protein is exhibiting any kind of structural similarities with other proteins available in the PDB. How I...

01 October 2017 7,058 4 View

Can any one suggest me how to compare the active sites of two different proteins?

I am trying to design multi-target directed ligands. I have found some similar common ligands for both proteins. When I docked these ligands I have seen the score is almost similar. But these...

08 March 2017 1,898 2 View

B factor comparison?

18 December 2016 3,013 3 View

Problem with L929 cells?

After 3days of splitting I am facing turbidity problem in L929 cells(Fibroblasts),But there are no symptoms of bacterial contamination.. Some protocol says to isolate conditioned medium from L929...

09 October 2016 4,451 2 View

Does anyone know how to train a matlab custom neural network in reinforced manner(Only using "adapt" function)?

09 September 2016 1,668 3 View

I have loaded dna in 0.8%gel at 85 v i saw the band after 45 min the band gone ..do dna run fast on 0.8% gell

crude dna size will be large know then how could it ran fast on 0.8%gel KIDNEY TISSUE DNA I have isolated and loaded to the well

26 April 2016 9,823 19 View

How to synthesize 4-sulfopthalic acid from phthalic acid at lab scale

Dear all, I want to synthesize sulfophthalic acid from Phthalic acid so i need a scheme of reaction to carryout this synthesis

16 February 2016 5,586 1 View

Any tool for Speech to Text conversion especially for Indian English?

I have tried working on Kaldi and CMU's Sphinx, these work well for US accent however the accuracy for Indian English is not good. To train the model with Indian English there is a need of...

18 August 2015 6,857 3 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

How to measure cosine, abbe, deadpath errors?

Dear sir, How to measure cosine, abbe, deadpath errors. I know cosine error, abbe error, deadpath error occurs in laser measurement. But how i can identify the cosine error value. Is any separate...

03 February 2021 4,878 4 View

How to apply graphene oxide on an gold electrode?

Dear Fellows, now it is the time to push graphene applications further in terms of easy and low cost production surroundings. Thus I recently dived in the materia and I am facing tons of papers,...

02 February 2021 6,284 2 View

Which method is preferable for cutting the nanocomposites?

Which of the methods like water jet cutting, diamond cutting, laser cutting, etc. is more suitable to cut the carbon nanotubes reinforced epoxy composites into test sample to ensure the clean...

29 January 2021 1,354 5 View