Which is the first restriction enzyme to be discovered Hind II or Hind III (As there has been lots of controversies over it)?
According to this article, it was actually a mixture of HindII and HindIII:
Article Highlights of the DNA cutters: A short history of the restri...
"The first of these new enzymes, HindII, was discovered in Hamilton (‘Ham’) Smith’s laboratory at Johns Hopkins Medical School in 1970 (29). ...Purified from Haemophilus influenzae serotype d, HindII (originally called endonuclease R) was found to act as a homodimer and to cleave DNA at the symmetric (though degenerate) sequence GTY’RAC (Y = C or T; R = A or G;’ indicates the cut site) (29,30). Subsequently, what was thought to be pure HindII was found to be a mixture of HindII and a second REase made by the same bacterium, HindIII. HindIII cleaved DNA at a different symmetric sequence, A’AGCTT (31,32)...."
Interesting question! Hind II discovered at the Johns Hopkins Medical school in the Hamilton Smith's laboratory in 1970.
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