PCR- 5,8S ITS and RFLP could be a rutinary method to identify yeasts. But not always you will arrive to the species, so I suggest you to do a PCR of the D1/D2 26S rRNA and then sequencing the PCR product at Macrogen Korea Facilities and comparing rRNA sequences from the GenBank database. Follow the protocol of Kurtzman and Robnett. Antonie van Leeuwenhoek 73: 331–371, 1998.