way of preservation blood (plasma) for RNA extraction less than 24hr is there any effect ?
incase I want to keep it for 3day before extraction which is the best way for preserving
Hi Raya
keeping safe samples in tubes during days is never good for further analysis. the best way therefore in your case is to use tubes as the PAXgene tubes from BD, but it's expansive:
https://www.bdbiosciences.com/en-us/products/blood-collection/blood-collection-tubes/paxgene-blood-rna-tube.762165
all th ebest
fred
Thanks frederic
but like this tubes not available in iraq
what about using rna shield ,trizol, rnalate??
rnashield and trizol is available
is there any effect on quality of results
I don't know rna shield but it's sold to keep samples at room temp (4-25°c) for 30 days, 3 at 37°c.
it depends therefore on your weather, a key point I didn't notice.
rna shield should therefore be fine.
all the best
Thanks a lot
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