I have tetracycline, pentaglycine and hexaglycine, and I need to make an aqueous stock solution of them. Dissolving in MiliQ gives a cloudy solution and precipitates out after some time. Can someone suggest any alternative way to make a 50mM stock solution of them without using acids or organic solvents as suggested on company websites? I need a clear solution for them at near-neutral pH.
Deep eutectic solvents (DES) have been reported to be good solvents for peptides. For example
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One typical component of DES mixtures is choline chloride, which is neutral. The second component can be acidic but need not be - for example, urea, glycerol, other glycols or polyols. You can make your stock solution of peptide in a DES of your choice and then dilute an aliquot with water. Of course if the peptide has very low solubility in water it will eventually reprecipitate. I think this is not the case with your glycine-based peptides.
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