I know the Hydroxylamine and NBT methods to determine superoxide Ion. But, which is the most accurate method to avoid the rapid disappearance of the superoxide ion in plant samples and to adequately detect its presence?
Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...
26 January 2021 7,672 2 View
Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...
11 January 2021 6,005 4 View
In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?
15 December 2020 8,231 14 View
There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...
18 November 2020 7,650 6 View
Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?
07 October 2020 739 3 View
Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...
03 July 2020 4,487 7 View
02 July 2020 5,724 13 View
Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...
22 June 2020 3,474 5 View
I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...
21 May 2020 6,692 1 View
As shear strength of AA2024-T4 alloy is inversely proportional to the temperature. is there any equation to predict shear strength of AA2024-T4 with respect to temperature?
12 May 2020 8,804 2 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...
15 February 2021 5,514 4 View
I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...
26 January 2021 4,542 2 View
Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...
25 January 2021 8,333 3 View
I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...
20 January 2021 1,542 4 View
I used percolation columns to reclamation saline-sodic clay soils . And my question is, What is the difference between soluble ions and exchangeable cations?
18 January 2021 6,868 3 View
Hi, I was curious to know what factors affect the resolution of a quadrupole mass analyzer. I find a lot of electrical settings that may affect the mass of the ions that are being transmitted. Is...
17 January 2021 5,724 1 View
I'm a new siesta learner, I'm working on lithium-ion batteries, I have calculated adsorption energy but i am not able to calculate diffusion barrier of Li ion. kindly help me how to calculate...
14 January 2021 2,688 1 View
I want to test the chloride ion concentration in the soil sample where I am conducting a research, but I cannot find a procedure to proceed. Thanks in advance!
07 January 2021 5,646 9 View