I know the Hydroxylamine and NBT methods to determine superoxide Ion. But, which is the most accurate method to avoid the rapid disappearance of the superoxide ion in plant samples and to adequately detect its presence?
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
Which membrane material is the best for capturing and visualizing phosphorylated proteins in a western blot? Or is there no difference?
05 June 2024 9,502 2 View
Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...
03 June 2024 6,795 1 View
Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...
08 May 2024 5,665 0 View
I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...
06 May 2024 8,974 5 View
Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...
11 April 2024 4,457 1 View
There are significant challenges that the blood bank industry in Kenya must overcome in order to be able to maintain a balanced equilibrium between the demand for blood and the supply of blood....
10 April 2024 974 2 View
Based on the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan, et,al. (2023)
09 April 2024 1,796 1 View
I wonder if there has been a progress in the way that conservators treat the metallic threads, especially the ones that are associated on liturgic textiles.
17 March 2024 4,773 0 View
Esta pregunta se refiere ha como podemos saber, si después de una intervención, ya sea en grupo o individualmente, a la hora de tratar con adolescentes problemáticos, nuestra intervención a sido...
11 March 2024 7,801 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,500 3 View
What is human-computer interaction (HCI)?
22 July 2024 10,056 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
Suppose a material "A" has both tetrahedral and octahedral sites and we are doping another material "B" - usually an ion into it. How can we detect if the dopant has occupied the octahedral site...
17 July 2024 4,299 4 View
I need to plot cycle number vs. capacity with multicurrent density. please let me know if my steps are right or not as the below picture
14 July 2024 7,702 2 View
What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
Hello everyone, I currently have a protein in a fully equilibrated membrane system. For the analysis I am running, I need to remove Na+ ions from the inside of the pore region. I would normally...
25 June 2024 4,579 4 View
Any help will be appreciated!! I am trying to use the Visual MINTEQ to investigate the speciation of ions in some environmental samples. In the input files, I only fixed the pH value and provided...
24 June 2024 9,138 0 View
What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?
24 June 2024 6,927 3 View