Hi, researchers.
I'll test a PCR-based method for detection of Mycoplasma hyopneumoniae in samples collected from benches and equipments (bioreactor and centrifuge). However, I need help to know which sampling technique is most appropriate.
Should I swab these surfaces? How?
Can anyone suggest what needs to be done in order to get representative samples?
Thanks!
Hi, Ani Amar!
I'm culturing Mycoplasma hyopneumoniae. So, for the next processes of production, I must certify that it's not growing in the bioreactor after cleaning and sterilizating the equipment and area. M. hyopneumoniae is my target.
I think I will use this kit for DNA extraction: https://tools.thermofisher.com/content/sfs/manuals/MAN0014333_PureLinkMicrobiome_EnvSwabs_UG.pdf
I also appreciate the suggestion of other method to extract the DNA! Thanks for answering me!
Hi.
I am not familiar with this Mycoplasma "friend". But you can perform:
1. Total viable count (for example rinse sample).
Then, filtration, incubation and identification. I also recommend you to give few time points for incubation (e.g., 72 hr, to check it, and then to continue if relevant (e.g., week= it depends on this "myco-friend" specific conditions).
2. But also you need LAL (endotoxins).
For swabs, you need to justify worst case locations in your equipment. I recommend you sterile COPAN swabs.
I understood that you know how to identify "myco-friend" colonies. Otherwise i missed something. I hope this helps.
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