Luis Felipe Buso Bortolotto

6 Questions 11 Answers 0 Followers

Questions related from Luis Felipe Buso Bortolotto

How can I sterilize nonfat dry milk solution and avoid caramelization?

I have been using a nonfat dry milk solution (100 g/L) for Salmonella pre-enrichment on samples of cocoa products. This solution is recommend by ISO 6887-4, instead of Buffered Peptone Water,...

26 August 2021 5,501 5 View

Which is the best method for sampling Mycoplasma from equipments and facilities?

Hi, researchers. I'll test a PCR-based method for detection of Mycoplasma hyopneumoniae in samples collected from benches and equipments (bioreactor and centrifuge). However, I need help to know...

28 May 2018 3,035 3 View

Is trans-chalcone strictly synthetic?

Many papers suggest that this compound is synthetic, produced by PGSS technique. Does anyone know if it can be naturally occurring? I need one example at least .

05 November 2014 6,145 3 View

Down-regulation of Bcl-2 and up-regulation of Bax and CIDEA. Does this ALWAYS mean apoptosis?

On my work, flow cytometry experiments with treated tumor cells point to cell cycle arrest on G1 and apoptotic death, but there's no p53 and caspase expression. PCR array experiments were done...

17 October 2014 3,321 8 View

Do tumor cells subjected to treatments with apoptosis-inducing drugs have up-regulated oncogene expression as a "defense mechanism"?

I mean genes like BFAR, IGFR1, etc.

30 September 2014 560 1 View

Is LDH assay able to evaluate possible mechanism of action related to necrosis?

Since LDH assay determines membrane integrity, may this test be sufficient to conclude that there is a necrosis cell death-related induced by drugs?

27 September 2014 6,937 8 View