I have analyzed my qPCR data by two methods. One by delta delta Ct method and another by normalizing the log 2 Ct of Target genes by the endogenous controls. I was expecting the same trend by either of the methods but surprisingly I got different trend for few cases (for others it was same by both the methods).
Jochen Wilhelm
"normalizing the log2 Ct" ?? Did you write this wrongly or do I just misunderstand it?
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Nidhi Sharma
Yes, there was some mistake in writing, so I am rephrasing it. I calculated the antilog of Ct values (for both endogenous and target), and then I normalized the target genes by endogenous controls and at the end from this normalized value I obtained the log (base 2) values.
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