Nidhi Sharma

5 Questions 9 Answers 0 Followers

Questions related from Nidhi Sharma

Is MAB3846 a suitable product for detecting HSP70 in Crassostrea gigas (Pacific oyster)?

I need an Antibody for detecting HSP70 B2 (An isoform of HSP70) in Crassostrea gigas (Pacific oyster). I ordered MAB3846 (mouse Ab) from Millipore. Its datasheet says that ''the epitope of clone...

02 February 2014 5,460 2 View

Which antibodies can identify the SDS-denatured protein?

I have a secondary and two primary antibodies in my lab (which can be used against actin and HSP70 in Western blotting). The application notes in their data sheets only says that they can be used...

11 November 2013 403 2 View

Which is the best method for analyzing qPCR data?

I have analyzed my qPCR data by two methods. One by delta delta Ct method and another by normalizing the log 2 Ct of Target genes by the endogenous controls. I was expecting the same trend by...

11 November 2013 5,014 2 View

Is 40% identity between the query sequence and the subject good enough for selecting antibodies?

I am looking for an antibody for HSP beta1 (small heat shock protein in Crassostrea gigas EKC40046.1). In mouse HSP beta1 is also known as hsp27, hsp25 (UNIPROT kb/SWISS-PROT: P14602.3). I aligned...

11 November 2013 8,025 2 View

Can any one tell me the best way to purify cDNA? Is it by column or precipitation with sodium acetate and ethanol?

I understand that it is not necessary to purify cDNA for real time PCR (as I had some good results previously) but still I want to be really sure.

10 October 2013 4,999 10 View