Purity on nanodrop for the plasmid isolated?

I isolated a plasmid (image attached along with), however the 260/230 ratio was less than desired i.e. 1.29 as compared to the ideal 2.0-2.2. Would it influence the digestion reaction?

09 November 2020 7,666 2 View

I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

What is the best buffer for plants to avoid osmotic shock?

Hi! I am doing an experiment where I need to spray a chemical on plant. I will be using autoclaved milliQ water as a base to dissolve small quantities of that chemical. I was wondering how can we...

09 April 2020 5,808 2 View

Does anyone uses Welch column 2.0 * 30mm, 30um for protein A analysis? If so, what is the column chemistry?

Hi, Does anyone uses Welch column 2.0 * 30mm, 30um for protein A analysis? If so, what is the column chemistry? Regards, Harleen

27 August 2019 4,767 3 View

What's the potential of metal enclosure of an LED luminaire not connected to ground with a Class 2 driver for providing input?

I have a 13 W, 24 VDC luminaire, getting input from a class 2 driver. I had a debate about whether dielectric test is required on the luminaire if the driver is CLass 2, UL listed. And, while I...

09 September 2018 5,894 2 View

Is there any human motor neuronal cell line available?

We are interested in examining the effect of CSF from MND patients on motor neuron cells.

29 March 2015 4,112 1 View

Kindly let me know what's the best way to qualitatively and quantitatively identify Menthol in a herbal formulation (herbal tea in granular form)?

Should it be GC-MS? Also, if we are testing same formulation's sample few months apart, menthol being volatile is liable to decrease with time? Yes / No How to maintain the stability of menthol in...

27 January 2015 1,687 6 View

Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?

Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?

04 November 2014 1,152 3 View

Oral Cancer Cell Lines for its treatment with Herbal Product

Dear All I need help..which Oral Cancer cell line would be best for its treatment with Active pharmaceutical Ingredient from herbal plant..And how can i get these cell lines in India and its rough...

01 January 1970 5,151 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View