I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

What is the best buffer for plants to avoid osmotic shock?

Hi! I am doing an experiment where I need to spray a chemical on plant. I will be using autoclaved milliQ water as a base to dissolve small quantities of that chemical. I was wondering how can we...

09 April 2020 5,808 2 View

How to know the concentration of compound in nanogram/Litre from a calibration graph in microgram/Litre?

I have calibration graph using GC-MS ion trap of a compound in microgram/Litres but i got the compound value in sample in nanograms/litre. Now i need to know the concentration of the compound. How...

01 February 2020 9,649 4 View

Why multiple bands of plasmid DNA extracted from unknown bacterial specie?

I extracted plasmid DNA from an unknown bacterial specie using NEB plasmid extraction DNA kit. After runnning the DNA on 1% gel i got multiple bands of the plasmid DNA. The last band of plasmid...

02 January 2019 3,408 3 View

Can anybody provide literature on horizontal cloud database partitioning with data mining?

I want to pursue my research work on database partitioning for scalable transactions in cloud storage.

19 February 2018 3,177 3 View

Is it a good topic for research: data fision:data partitioning in cloud computing?

What new is being done on this topic. Plz share some literature if anybody have.

18 February 2018 6,364 3 View

Is there any human motor neuronal cell line available?

We are interested in examining the effect of CSF from MND patients on motor neuron cells.

29 March 2015 4,112 1 View

Kindly let me know what's the best way to qualitatively and quantitatively identify Menthol in a herbal formulation (herbal tea in granular form)?

Should it be GC-MS? Also, if we are testing same formulation's sample few months apart, menthol being volatile is liable to decrease with time? Yes / No How to maintain the stability of menthol in...

27 January 2015 1,687 6 View

Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?

Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?

04 November 2014 1,152 3 View

Trying to amplify a gene from genomic DNA with primers with low GC content and attached restriction sites, what would be the optimum PCR conditions?

21 April 2014 2,977 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View