Inactivation of Restriction enzyme?

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ماهو دور انزيم الكاتليز في النباتات؟

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Ryan Wheeler

Some restriction enzymes will cut off-target sequences if they are allowed to incubate with the DNA for extended periods of time. I have never witnessed this personally, but it is cautioned against by RE manufacturers. See link below:

https://www.neb.com/tools-and-resources/usage-guidelines/star-activity

Fatemeh Khozaei

Dear Mehak Taneja

Generally, Heat inactivation is a convenient method for stopping a restriction endonuclease reaction. Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C. Heat inactivation was performed as follows to approximate a typical experiment.

Best Regards

Dr. Fatemeh Khozaei