Inactivation of Restriction enzyme?

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Purity on nanodrop for the plasmid isolated?

I isolated a plasmid (image attached along with), however the 260/230 ratio was less than desired i.e. 1.29 as compared to the ideal 2.0-2.2. Would it influence the digestion reaction?

09 November 2020 7,666 2 View

I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

What is the best buffer for plants to avoid osmotic shock?

Hi! I am doing an experiment where I need to spray a chemical on plant. I will be using autoclaved milliQ water as a base to dissolve small quantities of that chemical. I was wondering how can we...

09 April 2020 5,808 2 View

How to know the concentration of compound in nanogram/Litre from a calibration graph in microgram/Litre?

I have calibration graph using GC-MS ion trap of a compound in microgram/Litres but i got the compound value in sample in nanograms/litre. Now i need to know the concentration of the compound. How...

01 February 2020 9,649 4 View

Why multiple bands of plasmid DNA extracted from unknown bacterial specie?

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02 January 2019 3,408 3 View

Can anybody provide literature on horizontal cloud database partitioning with data mining?

I want to pursue my research work on database partitioning for scalable transactions in cloud storage.

19 February 2018 3,177 3 View

Is it a good topic for research: data fision:data partitioning in cloud computing?

What new is being done on this topic. Plz share some literature if anybody have.

18 February 2018 6,364 3 View

Is there any human motor neuronal cell line available?

We are interested in examining the effect of CSF from MND patients on motor neuron cells.

29 March 2015 4,112 1 View

Kindly let me know what's the best way to qualitatively and quantitatively identify Menthol in a herbal formulation (herbal tea in granular form)?

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Hello, does anyone have any experience with packing columns for nano LC? Any suggestions on the tubings and the method used for column packing?

04 November 2014 1,152 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

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21 February 2021 5,379 2 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

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Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

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ZnS oxidizes to ZnO!!why??

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How long does it take to design and validate transition state analogue inhibitors?

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Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

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Kinetics of Enzyme production in Recombinant E Coli?

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Why non-specific band appear on T-ARMS PCR?

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09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

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08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

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Ryan Wheeler

Some restriction enzymes will cut off-target sequences if they are allowed to incubate with the DNA for extended periods of time. I have never witnessed this personally, but it is cautioned against by RE manufacturers. See link below:

https://www.neb.com/tools-and-resources/usage-guidelines/star-activity

Fatemeh Khozaei

Dear Mehak Taneja

Generally, Heat inactivation is a convenient method for stopping a restriction endonuclease reaction. Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C. Heat inactivation was performed as follows to approximate a typical experiment.

Best Regards

Dr. Fatemeh Khozaei