Which is best secondary antibody Alexa flour 488 labelled or dylight 488?

More Binney Sharma's questions See All

Do anyone here can help us in the field of molecular docking?

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01 March 2021 1,899 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Modeling viscosity using model of Chung?

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25 February 2021 8,053 5 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

RDF calculation of water inside the Carbon nanotubes?

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17 February 2021 1,066 3 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

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16 February 2021 3,796 9 View

I am looking for a microbend optical sensor design...can anyone please provide me some idea about it??

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11 February 2021 5,911 2 View

How to interface ANSYS Maxwell and Matlab?

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10 February 2021 1,279 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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Issues with Permeabilization during Flow Cytometry?

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Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

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01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

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28 February 2021 1,577 1 View

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RIA. How to measure?

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Anyone know of a good progesterone antibody?

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How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

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Peter Bou Dib

they will both do the job. optimize your staining protocol and you won't be able to tell the difference between them.

Good luck!

Chris Langsdorf

Hi Binney,

I agree with Peter - optimization is key. You may also want to check out new Alexa Fluor Plus secondaries. Updated conjugation technology gives better S/N.

Chris