Which is best possible conjugate for preparation of polyclonal antibodies against melatonin

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ELISA and Hybridoma

Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.

07 August 2017 2,293 5 View

Western Blotting

Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?

07 August 2017 9,508 1 View

All immunogens are antigens, but not all antigens are imunogens.?

Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”

07 August 2017 4,712 7 View

ELISA and Immunohistochemistry

Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?

07 August 2017 945 5 View

ELISA and Hybridoma Technology

An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...

07 August 2017 8,415 7 View

ELISA

I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...

07 August 2017 5,462 4 View

ELISA, Immunoprecipitation and Affinity Chromatography

What are some common treatments that could be used to disrupt the antibody-antigen complexes?

07 August 2017 5,565 4 View

Western Blotting and ELISA

What is the function of the enzyme bound to the secondary antibody?

07 August 2017 8,198 9 View

Dilution of Samples in ELISA

What is the need for serial dilution of the sample when performing an Elisa?

07 August 2017 1,692 1 View

Western Blot, ELISA and Immunohistochemistry

What are the differences and similarities between western blotting and immunohistology?

07 August 2017 2,178 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

Why don't my intestinal organoids from children's polyps grow?

Hi! I am having trouble growing intestinal organoids from children's polyps. Sometimes they don't grow at all, and other times they look dark and unhealthy, as shown in the attached pictures...

18 June 2024 4,781 3 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Do plants produce hormones that have the same influence properties of animal hormones?

I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?

13 June 2024 3,860 1 View

How many tyrosine concentration we should add for increasing product?

Adding substance like amino acids such as Tyrosine or L-tyrosine in E.coli and LB broth, How many concentration we should add for increasing product?

11 June 2024 5,512 2 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View