How many tyrosine concentration we should add for increasing product?

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Michael Kohlstedt

Dear Patch,

I would start with 200 µM and measure whether it improves growth and/or production and check whether it is taken up or not. Maximum concentration tyrosine dissolves properly in aqueous solutions is less than 2 mM:

https://www.sigmaaldrich.com/DE/de/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/l-tyrosine-in-cell-culture

Best

Michael

Patch Porit

Thank you for your respond, but I'm still have question. Do you have any suggestion about adding tyrosine with culture media for feeding E.coli?