Which is an appropriate column to be used for the analysis of nucleotides like thioguanine mono/di/tri phosphates for LC-MS ?

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Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

What is the minimun number of studies for each subgroup analysis in meta-analysis?

Hello everyone, I am conducting a meta-analysis to find if x has superior effect than y on the outcome. I used the SMD method and I got a significant moderate heterogeneity of a value I2:...

09 June 2024 8,446 3 View

Effect size to check if x is superior than y... How to calculate it? within or between?

Hello everyone, I am conducting a systematic review to see if treatment x is superior in its effect than treatment y. Since most the studies I found did not report interaction effect, I was...

24 May 2024 6,130 2 View

What are recent UWB Antennas ?

I require recent UWB antennas manufactured

15 May 2024 3,888 1 View

Which is the best free software for RAPD data analysis?

I am searching for a free best software for RAPD data analysis and phylogenetic tree construction

09 May 2024 7,017 3 View

Is there a way to combine CFA with linear mixed model taking into account random effects?

I try to explaim better. I have the scores for 10 items. Each item score are decided between the accordance of two persons (say, e.g., a therapist and a patient). Therefore, there is much more...

24 April 2024 4,563 6 View

Difference between Henderson and REML method of combining ability estimates?

I want to know whether there will be difference in the results obtained in AGD-R software of CIMMYT when Henderson and REML methods used in the balanced data.

21 April 2024 5,361 0 View

How do I interpret Hedgs g sign? How do I calculate effect size when only median with CI is reported ?

Hello everyone, I am writing a systematic review about if X experimental group has superior effect than Y comparison group on outcome. Please note that the lower the scores of the outcome, the...

19 April 2024 1,812 1 View

Methods to calculate Shannon Index?

I have three field margin types (grassy/shrubby/tree) and each type have 5 sites. I need to calculate Shannon index for insect counts from each field margin type.... Q1. In my case how should I...

19 April 2024 681 6 View

How to fix the error during importing Gas Phase reaction, surface reactiona and thermodynamic data Chemkin file in fluent?

Hello All, Greetings The issue I am encountering pertains to the Chemkin files to be uploaded into the Ansys Fluent software. Unfortunately, I am encountering persistent errors, and it seems the...

04 April 2024 8,189 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View