I am looking to conduct a proteomic study and need a cell line that can grow in serum free medium. Also is there a defined medium that is ideal for mass spec analysis such that the medium contents don't interfere with the MS results.
You can study with CHO and BHK 21 cell line. They can easily adapt to serum free media and easily grow.
ooo yeah, I'm overlooked. Human cancer cell line easily grown serum free media, especially human breast cancer and colon carcinoma cell line.
u may use Hela cells they jst grow like weeds ,they grow well in DMEM media or RPMI and donot innterfer with MS.
Thanks guys. Do you know of any primary lines that might grow ok in serum free conditions or chemically defined medium? I know primary cells are finicky but if anyone has experience with growing them in defined conditions, that would be useful info.
primary cell lines are preety sensitive they tend to form spheroid bodies in serum free media or low serum media, but one thing that can be done is u can supplement the media with essential growth factors like insulin , egf etc but keep in mind this growth factors are too costly , can increase your your cell culture costs is large scale.
all d best..!
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