Hi,
I'm conducting a bacterial microarray expression data analysis on RStudio and have got 3289 and 59 differentially expressed gene ID's while using an FDR value of 0.05 and 0.01respectively. But, I have found only one wet-lab experiment (based on microarray analysis and PCR result) to validate my result. Moreover, When I used the FDR value of 0.01, I got almost the same result as the previous wet-lab experiment. But, as far as I know, most of the researchers prefer the FDR value of 0.05 to screen out differentially expressed genes.
Does anyone have a clear explanation about it?
Thanks
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