I'm a student who is learning bioinformation analysis these days. As mentioned above, if there is only 1 sample in each group in a microarray datasets obtained from GEO, how can I find out the significantly expressed genes? Is there any simple and affordable methods to solve this problem? Thanks a lot.
I'd leave it. n=1 does not give you any hint about the biological variation. This might be inferred from other experiments (e.g. also obtainable from GEO), but there are so many possible pitfalls and I have never seen anyone doing this.
Hi Chrisperez
yes it's possible, but due to all bias we met (in microarrays, NGS...), statistics will give you whatever it wants since you don't have enough power to run paper analysis. so, no, it's impossible to get reliable results in this condition, you should take Lochem's advice into account, it's far away better.
all the best
fred
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