How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Where can I find solar irradiation data with high resolution?

I am trying to generate a 1 day solar irradiation profile. Any suggested high resolution(possibly in minutes) source for the same?

06 December 2020 5,976 18 View

How to implement only a Gate with activation sigmoid or tanh in keras?

I want to implement only a Gate similar to forget gate or output gate of LSTM cell. Mind it...!!! I am not implementing the entire LSTM cell rather I have certain input and previous output and I...

22 September 2020 8,236 3 View

Could someone help me interpret the following PMF curve, especially the physical reason behind the multiple potential wells?

I am simulating the pulling of two nanoparticles in a polymer. I get the attached PMF curve, showing a secondary (and much deeper) potential well. I was wondering if there is a physical...

19 September 2020 7,504 5 View

How to determine relationship among the independent variables to get lowest MSE (Mean Squared Error) in prediction?

I have a dependent variable A, which can be perceived from B, C and D. So to say A = function(B,C,D). B, C, D results in A that is known to us. However, I don't know whether B,C and D follow a...

28 June 2020 6,332 6 View

IS it Necessary for Primers in Taq-Man PCR to be HPLC Purified?

I want to set up a taq man reaction. I want to know that in taq man reaction I have to order HPLC purified Primers or ordinary primers are adequate for me. If you have experience in taq man,...

23 June 2020 5,511 3 View

No title

We're looking at storing serum and plasma samples from a clinical trial. I've looked at various papers, but the only place where I found some hazy answer was in the supplementary. But I'm hoping,...

04 March 2020 8,858 3 View

How to calculate the required motor HP to run a machine?

Hello every one, Can some body help me in calculating the required HP for motor to run the machine which includes transmission system with 2 Nos of shaft and cutting blades.

28 December 2019 9,788 3 View

Is there any benchmark dataset for distributed camera networks?

I am particularly looking for dataset containing cameras capturing videos in a distributed setup. The videos, captured by multiple cameras have to be correlated. Is there any benchmark dataset on...

22 November 2019 9,567 3 View

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

01 August 2019 8,465 4 View

Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Sample Size Specifics - Does it matter for quantiative studies?

If I'm specifically stating that I want the sample size to be set at 32, for example, does that alter or change my study? It is a quantaitive study utilizing an experimental pretest-posttest...

04 May 2019 4,487 3 View

Is Multitasking possible?

Is multitasking is possible:- Yes it is possible with the help of balance of doing the things, with in small quantity or quantum or in bits and pieces like if we believe that today is our last day...

02 March 2019 6,695 3 View

Does anyone works memory tests in OpenSesame software?

24 April 2018 3,878 2 View

Does anyone works memory tests in OpenSesame software?

03 April 2018 2,916 2 View

Does anyone have the actual items for the MMI - media multitasking index questionare. The papers all wrote as if it's obvious and available but?

Does anyone have the actual items for the MMI - media multitasking index questionare. Orphir, Nass & Wagner. Or know where to get them? The papers all write as if it's obvious and available but I...

02 March 2018 7,699 1 View

Do you know any research about biofeedback as a way to reducing level of informational stress?

02 March 2017 4,836 0 View

Someone knows if there is any good package in R to program psychological tasks?

I am aware that there are several programs such as Presentation, E-Prime, OpenSesame, even Matlab that can easily be used to program tasks. However, considering that all these programs are not...

10 November 2016 7,378 2 View

What best software is recommended for an academic researcher to conducting a meta-analysis ?

07 May 2016 4,429 7 View

Do you know any research about connection between media multitasking and depression?

As I wrote in the title of this question, I would be very grateful for any hints, papers and ideas about conncection between high media multitasking and states of depression, anxiety - this kind...

11 December 2015 5,650 1 View