Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

Which filter size should I use for sterile-filtration of aqueous glycerol solutions (10%)?

Hi everyone, I heard that glycerol is better sterile-filtered than autoclaved. Can anybody tell me the appropriate filter size? Thanks in advance!

29 April 2018 5,626 3 View

Studies of wavelets with dimension?

Are there any famous and easy to read, or benchmark study on wavelets, where they use dimensions for time and frequency? If the amplitudes have a correlation to spatial coordinates, for any...

19 May 2017 6,242 1 View

HV Transmission pole (possible scaling and function?)

14 February 2017 7,845 3 View

How many ug of exosomes are needed for EM analysis?

Hi, I am planning to do EM analysis on exosomes derived from neural progenitor cells. I have quantified the amount of exosomes that I got from my prep by BCA assay and it gave me a concentration...

05 February 2017 7,119 1 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View