Which equilibration buffer be used to equilibrate protein cleanup spin column of AllPrep RNA/Protein kit (Qiagen)?

10 October 2014 1 1K Report

They didn't supply the equilibration buffer. However I need to equilibrate the column so which buffer could be used? I am planning to do western blot.

Francisco Solano

As far as I know, there is no particular requirements. Phosphate, Tris. Concentration should not be very high. You can use a buffer similar to the sample buffer for electrophoresis without dyes.

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