Prem Prasad Lamichhane

4 Questions 8 Answers 0 Followers

Questions related from Prem Prasad Lamichhane

How can I dissociate primary dendritic cells for flow cytometry?

I am going to perform flowcytometry of dendritic cells cultured from PBMC. How can I dissociate the adhered cells? Can we trypsinize? How much percentage trypsin is required? Is there any...

11 November 2014 2,302 9 View

Is it necessary to wash cells with PBS prior to addition of TRIZOL?

I am going to extract RNA from influenza virus infected cells using TRIZOL. So, I wonder whether culture media will have any interference in RNA extraction and subsequent downstream application.

10 October 2014 8,142 5 View

Which equilibration buffer be used to equilibrate protein cleanup spin column of AllPrep RNA/Protein kit (Qiagen)?

They didn't supply the equilibration buffer. However I need to equilibrate the column so which buffer could be used? I am planning to do western blot.

10 October 2014 959 1 View

Where can I buy human dendritic cell lines?

Not the primary cell.

06 June 2014 9,334 4 View