I'm pretty sure there aren't any.... sorry. If you find them, please let us all know.
However it is straightforward (although tedious and not inexpensive) to isolate them directly from human peripheral blood. StemCell Technologies make a great kit for negative isolation of human pDCs, for example. Also, monocyte-derived DCs are simple and relatively inexpensive to make, again from human peripheral blood.
Is there a specific reason you need a cell-line?
Good luck
G
As stated above, I think you could treat human monocytes with GM-CSF and IL-4. It takes about 5 days to observe dendritic cell (DC) features in those monocytes. They start to express CD1a an lose the expression of CD14 (as seen in natural DCs) and also display morphological features of DCs. There are several papers in literature where you may find such information.
In case you are looking for DCs available from a commercial source.
Please refer to NHDC-Normal Human Dendritic Cells, in LGM-3™ from LONZA.
Hope this helps.
There are several DC-like human cell lines that have been used for various experiments. Each has their own deficiencies and as with any immortalized or transformed line there are always questions regarding its ability to represent specific functions associated with primary cells. That being said some DC models are very useful for preliminary experiments on signaling pathways or as a ready source of APCs for in vitro or in vivo stimulation of T cell responses. The human conventional DC-like lines used commonly are Thp-1, HL-60, U937, KG-1, and MUTZ-3. All of these, with the exception of MUTZ-3, are available from the American Type Culture Collection (ATCC.org). There is also a plasmacytoid DC like cell line, PDMC05. I have attached a review that evaluates several of these DC-like cell lines.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology