What could happen if I stop a PCR reaction to add a component I missed out during the mastermix prep?

Let's say I forgot to add the polymerase enzyme and I only realize this after the run has gone half way, could I stop the reaction to include the pol...would I get amplification if I reprogram...

13 September 2019 3,211 3 View

Can heparin tubes be used to collect blood samples for Plasmodium falciparum microscopy?

I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.

23 April 2019 8,803 4 View

How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

17 April 2019 2,270 4 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

19 June 2018 4,721 2 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

24 September 2017 1,928 2 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

24 July 2016 507 5 View

Why is the concentration of ammonia in my reactor is decreasing while nitrite is increasing?

I am trying to establish anammox bacteria to coexist with ammonia oxidizers. Can someone explain to me why the concentration of ammonia in my reactor is decreasing while nitrite is increasing...

19 January 2015 9,816 7 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

Tick-borne encephalitis (TBE) incidence in the Czech Republic in recent years?

Dear Sir or Madam, i'm a medical student. In range of school lesson i have to research about TBE (see topic) in the country of Czech Republic. I would be pleased if you could give me a hand on...

24 January 2021 5,984 4 View

The First Survey Addressing Patients with BMIs over 50?

Dear Bariatric Surgeons, The importance of obesity management in patients with BMIs over 50 has not been completely addressed and it should be considered separately from patients with morbid...

20 January 2021 2,748 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What is role of chronopharmacology or circadian rhythms in metabolic diseases including obesity, diabetes and cardiovascular diseases?

Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...

18 January 2021 9,045 3 View