Abdul Waheed Khan

6 Questions 11 Answers 0 Followers

Questions related from Abdul Waheed Khan

Transfection with CRISPR using turbofectin?

Dear all! we are trying to knockdown a gene in lymphoblastoid cell line using Origene CRISPR/CAS9 kit with the HMR method using the recommended turbofectin reagent. However, we are not getting any...

10 October 2018 8,932 3 View

Single cell isolation post CRISPR/CAS9?

Hi, I performed CRISPR/CAS9 genome editing in HL-60 cell line (suspension culture) to modify a gene of interest for a desired mutation (I used the sgRNA for my gene and a single strand ultramers...

04 April 2018 3,267 2 View

My phylogenetic tree topology is fine but the confidence (nodes bootstrap value) is quite less than the threshold. Do journals accepts such trees?

ML tree HCV NS5B sequences

03 March 2017 7,458 4 View

Dear all ! I am getting the issue of plasmid self ligation during E. Coli transformation every time. How can I minimize the problem?

using pjd301 plasmid, 5UTR insert and DH5-alpha strain

12 December 2016 2,947 7 View

Which DNA sequences should i select in phylogenetic analysis as i am getting very low bootstrap value for phylogenetic tree using ML method?

HCV Evolution

01 January 2015 3,012 4 View

Which region or gene should be the most appropriate for phylogenetic analysis?

Criteria for selection of a gene for phylogeny - let it be the case of HCV ?

05 May 2014 6,611 3 View