Which dialysis buffer suitable to use in dialysis (protein sample)?

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Mahesh Chandak

Use the buffers in which your protein is stable, you have to read previous papers

Achim Recktenwald

If possible, do not use a buffer with high ionic strength, like 100mM phosphate, or 20mM phosphate, 500mM NaCl, etc.

If your sample carries over lots of salt into the sample prep for SDS-PAGE, you will get funny lanes. In extreme cases, the look very broad at the top of the gel and disappear into a thin line at the bottom.