Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

How to generate pulsed laser from CW laser?

I was wondering if it could be possible to modulate a CW laser to pico/nano seconds pulsed laser somehow. I need to generate that condition to pump DFB polymeric lasers. Probably using...

16 November 2020 7,601 3 View

Which dose do you expose RAG1 deficient mice for lethal-irradiation?

Hello, I'd like to apologize for my English in advance, I'm still trying to polish it. We are performing several bone marrow transplantations (BMT) or fetal liver transplantations (FTL) to...

05 November 2020 4,239 1 View

Recommendations for Fluorescent Western Blotting?

Hi everyone, we're planning to do some protein quantification using western blot. Since a lot of our proteins of interest are of similar size, I would either have to strip and reblot a lot or...

04 November 2020 5,033 2 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

Grounded theory or Interpretive phenomenological analysis? Which is best for dissertation studying perinatal mental health?

I am curious what the differences are between using grounded theory or interpretive phenomenological analysis (IPA) for my dissertation. I am seeking to both complete my dissertation for...

29 October 2020 3,066 4 View

How to calculate grain size if FWHM of Lorentzian contribution is nearly zero and lower that FWHM of instrument?

I want to calculate grain size and microstrains. I fitted my peaks with a Voigt function but the FWHM of the Loretzian contribution resulted to be nearly zero, lower than the FWHM obtained from...

28 October 2020 2,578 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View