Please clarify your question. Are you inquiring about volatile buffer options for use in LC-MS analysis? If so, please read the attached linked article, "Popular LC/MS and HPLC Volatile Mobile Phase Buffers".
http://hplctips.blogspot.com/2014/06/popular-hplc-volatile-mobile-phase.html
Hi,
I guess you're referring to detergents that can be used during preparation of samples for MS? In that case, there are some acid-cleavable detergents available (such as Rapigest) that could be worth looking into.
Good luck!
I think that you may find this Wikipedia article useful. Make sure to follow the links in the references.
https://en.wikipedia.org/wiki/Cleavable_detergent
If you really are looking for a digest for proteomics (question clarity needed), then something like Invitrosol works well, but remember that post digestion you still need to spin down and dry the material before reconstituting in mobile phase.
I‘m sorry that i didn't describe the question clearly. The detergent is added into lysis buffer and then i would purify proteins with flag agarose. Then i would use this sample to do a Mass Spectrometry.
Hi Zhang,
I've had good success adding 1% octylglucoside (a.k.a. Octyl β-D-glucopyranoside) to my lysis buffers, and then removing it post-digestion with an ethyl acetate extraction prior to MS analysis. Here is a paper describing the extraction method:
https://www.ncbi.nlm.nih.gov/pubmed/20155730.
This is assuming that your downstream MS analysis is bottom-up (and hence includes a protease digestion), not top-down. Good luck!
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