Which database do you recommend for AMF DNA sequence analysis obtained with primer pair NS31/AML2 ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to develop an academic literacy program for engineering at the higher education level?

Information literacy in higher education integration with curricula engineering

04 August 2024 5,368 3 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What should be the best Lumens range for T8 (120cm) full spectrum LED lamp tubes?

Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...

11 July 2024 6,078 1 View

Cross Attention in Transformers: Standard applications of the same ?

What are the standard applications of Cross Attention in Transformer Architectures ?

09 July 2024 9,310 2 View

Time Series Analysis: Has Recurrent Neural Networks (RNN) ever been used on Time Series Analysis ?

Are there standard RNN architectures been applied for Time Series Analysis, forecasting and anomaly detection problems ?

30 June 2024 3,169 8 View

LSTM on Time Series: Has LSTM architectures ever been applied to Time-Series Forecasting ?

Have we ever used LSTM architectures on Time-Series Forecasting and Analysis, and gotten a decent result ?

30 June 2024 6,924 3 View

What could be causing these smears in my PCR electrophoresis gel?

I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...

30 June 2024 4,107 4 View

What are the typical applications of Large Vision Models (LVMs) ?

Where are large vision models typically used ?

25 June 2024 4,113 0 View

Are there standard libraries/frameworks for doing RLHF for training LLMs ?

When it comes to Re-inforcement Learning with Human Feedback, are there standard libraries/frameworks for training LLMs ?

25 June 2024 1,121 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View