How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Why the bandgap of CH3NH3PbCl3 films are different for spin-coating method and electrospinning method?

The observed bandgaps are 3.8 eV and 3.4 eV for spin-coated and electrospun films, respectively. What are the possible reasons?

08 January 2021 5,646 6 View

Why monoethanol amine is used as flocculant?

I want to know the reaction mechanism of monoethanol amine used as flocculating agent for rerefining used lubricating oil. Here I have attached one research paper where MEA (monoethanolamine) is...

01 January 2021 5,681 2 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View

How can I determine metal concentration of Used lubricating oil by AAS?

I am a junior research fellow in the department of Chemical Engineering, Nit Durgapur. I want to know that how can I determine various metal such as Fe, Cr, Mg, Mn, Pb concentration present in...

15 December 2020 9,887 3 View

Regarding the O 1s XPS pf phyllosilciates, the peak near 531 belongs to Oxygen vacancy sites or O bonded to the Si from the silicate tetrahedra?

In O 1s spectra, usually the peak near 531 eV belongs to oxygen vacancy sites in oxides, however, in silicates, these peaks are often reported to originate from O-Si. I am confused if the intense...

10 December 2020 9,496 2 View

What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How to PEGylate Fluorescntl Polystyrene Particle?

I have Fluorescent Polystyrene beads. I need to graft PEG on the surface of it. I am looking for a protocol to do that. Also need to know which experiment to perform to be sure about the grating.

01 December 2020 7,860 1 View

How to control for categorical independent variables (e.g., education) when doing regression discontinuity using STATA?

I am conducting a regression discontinuity design where the dependent variable is continuous while control variables are categorical (except the treatment variable). In the usual regression...

01 December 2020 583 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View