Ripon Sarkar

6 Questions 5 Answers 0 Followers

Questions related from Ripon Sarkar

What should be the cell density for subcutaneous injection to induce tumor in BALB/c mice?

Hi, What cell density will be good to generate breast cancer in BALB/C mice? I am planning to use serum-free culture medium to suspend the cells for injection. Please recommend the best...

04 October 2021 1,508 3 View

How to troubleshoot Human umbilical vein endothelial cells (HUVEC) attachment?

Hi, I have been facing problem with HUVEC attachment. I culture the cells on 1% gelatin coated cell culture flask. After 60-70% confluence, cells layer starts detach from the surface. Even fixing...

03 September 2021 7,461 0 View

Which culture medium is best suitable for the differentiation of Dental pulp stem cells to neuronal lineage?

I am trying to differentiate primary DPSC to neuronal lineages. I used Neuro basal medium (Gibco) with growth supplement. After 7 days culture in that medium, cell are getting dissociated. I got...

04 October 2019 4,050 0 View

Why do primary cells form vacuole-like structure after passage no. 1?

I am working on normal primary oral keratinocyte cells. The cells have been isolated from enzymatic digested explant method. Cells were healthy after first split (passage=1), however, after second...

28 August 2017 415 2 View

How to design primer?

I want to study the mRNA expression level. I downloaded the FASTA sequence from NCBI. Then, I used online Primer designing tool from Thermo Fisher to design primer. I have confusion whether my...

25 February 2016 8,220 3 View

Can anyone suggest the protocol for protoporphyrin extraction from human cells?

I am going to extract protoporphyrin from human cells. I followed two extraction methods from publised articles but the results are not satisfactory. Can anyone suggest a proper extraction protocol?

11 February 2016 5,267 3 View