in my MTT assay,IC50 for drug was determined 43 µg/ml (in cell line). I want to analysis the effect of this drug in expression of 4 genes. Which concentrations of drug should I use? Less than IC50 or more? Should I use 1 concentration or several for each gene?
Hello Jebelli.
I want to know to which cell fate these genes are associated, are they related to cell death associated ..?
what actually are you aiming to determine through gene expression studies associated with these genes.?
if you would let that know i can help at what stage of MTT assay or at what concentration you can take the cells for study.
i want to determine whether this drug up or down-regulate the expression of these genes.
Dose response is the way forward to see the kinetics of the effect
0, 0.15, 0.45, 1.5, 4.5, 15, 45, in microg/ml
Dear Hasan,
Dear Yannis,
I am very interested by this topic.
Could you validate "more or less" your more than affirmative answers?
Some "validating" publications would be very helpful.
Thank you very much.
Robert