Which column and suppressor are most effective for separation of protein and enzyme using ion chromatography ?

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We are using ELICO HPLC , but there is the problem of getting peak of compounds ?

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Waters 2707 HPLC autosampler problem?

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How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

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Adam B Shapiro

For ion exchange chromatography, you can use either an anion exchanger such as Q-Sepharose, or a cation exchanger such as SP-Sepharose.

Sephadex G50 is not an ion exchange resin; it is a gel filtration resin. This can also be used for protein purification. For gel filtration using and FPLC, you should use a Superdex column.

Steingrimur Stefansson

Hi Ravi, this a very general question and there is no "one-size-fits-all" answer.

I'm sure that if you scour Google Scholar you will find a purification procedure for your enzyme or a similar enzyme.