Hello,
I am comparing the two products linked below: Pierce Cypridina Luciferase Flash Assay Kit and Pierce Cypridina Luciferase Glow Assay Kit. As I understand it, the only difference is that the Glow kit takes 10 minutes for the signal to stabilize and lasts longer than the Flash kit. Is the intensity of the Glow kit compromised at all due to its increased signal stability? Will the Flash kit give me a bigger window? Also, the protocol for both kits say that for detection of CLuc in the cell supernatant, supernatant should be moved to a separate plate and combined with reagent there. Do I need to move the supernatant? Or may I simply add the reagent directly to the wells in which the cells are growing? I understand this would consume more reagent. Also, for detecting CLuc, is using the supernatant better than the cells? I would prefer to use the supernatant because it negates the cell lysis step.
Thank you!
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