Murine cell lines are a common choice for testing osteogenic capabilities of a given scaffold.

Regards.

Nih3t3

Or you can use an human osteoblastic cell line:

hFOB 1.19 (ATCC® CRL-11372™)

http://www.lgcstandards-atcc.org/products/all/CRL-11372.aspx?geo_country=it

In addition to testing your material with bone-related cell types, you should test it with immune cells. Preclinical testing will have to address what would happen if your biomaterial entered the bloodstream (if your material is not a nanomaterial, and is unlikely to enter the bloodstream whole, then consider what will happen if fragments become detached).

It depends on what you exactly mean for biocompatibility.

For "basic" biocompatibility (is this material toxic for cells?) you can use just murine fibroblast 3T3 (easy screening). Then if you want to test the BIOACTIVITY of your material ( is this material able to induce a " positive response " in my cells?) you can use murine (MC-s3t3 preosteoblast or better human ehFOB or even bettegr mesenchimal cell hard to menage however) to test changes in enzyme expression and activity (e.g. alcaline phosphatase) or matrix deposition (a lot of dye to test this). If your material can induce an osteogenic response so it is a good idea to test other abilities (immuno, haematic etc)

You can use any cell line depending on the specific application of your material. But ISO prescribes L929 fibroblast cells/HeLa cells for the general biocompatibility testing of any biomaterials. Please refer ISO10993.

Dear Kübra,

You will find our articles below about this.

1. Synthesis and characterization of polymeric soybean oil-g-methyl methacrylate (and n-butyl methacrylate) graft copolymers: biocompatibility and bacterial adhesion.

Cakmakli B, Hazer B, Tekin IO, Cömert FB.

Biomacromolecules. 2005 May-Jun;6(3):1750-8.

2.

Synthesis and characterization of polymeric linseed oil grafted methyl methacrylate or styrene.

Cakmakli B, Hazer B, Tekin IO, Kizgut S, Koksal M, Menceloglu Y.

Macromol Biosci. 2004 Jul 14;4(7):649-55

Hi ROS or Saos are typical cell lines used in bone eng studies

see http://aubinlab.weebly.com/cell-lines.html

May I also suggest HOS (TE85) and MG63 cells, both available from ECACC (UK) - excellent human bone-like cell lines originating from human osteosarcomas.

Regards,

Vehid Salih

Thank you very much for your valuable answers.

Best regards,

Burcu

You can use L929 fibriblast cell of rats