Hello, everyone. I appreciate if anyone can answer my question. i want to analyze the cytotoxic ability of CD4+ T cells subset in vitro by CFSE assay. I need to know the appropriate cell line for this assay. k562, Duaidi, and NIH-3T3 ???
CD4 is expressed by Monocytes and macrophages, I have used U937 (human monocytes) and NR8383 (rat macrophages) cell lines before (for another purpose). Both cultures are easy and work very well.