I am planning to run some preliminary experiments to assess the expression of a Treg-like phenotype by checking markers such as FOXP3 and CD25. Could anyone recommend a suitable cell line for this purpose?
P.S- I don't want to use primary cell line now , as they are not easy to handle
Hello Mayank Singh,
MT-2 cells are used to study Treg biology due to their phenotypic and functional resemblance to human Tregs.
MT-2 cell line is an immortalized cell line, and these are human T cells that naturally express CD4 and high levels of CD25, as well as FoxP3, a specific marker for Tregs, thus exhibiting Treg-like phenotypic markers.
Another cell line which you could use to study Treg-like phenotypes, is the HuT102 cells, which display a Treg cell-like phenotype(CD4+CD25+Foxp3+). However, HuT102 cells can variably express Treg cell markers, depending on the cell cycle during harvest.
Best,
Malcolm Nobre do you have access to these cell line, I am from Mumbai, India.
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