Mayank Singh

5 Questions 2 Answers 0 Followers

Questions related from Mayank Singh

Which cell line is best for studying Treg-like phenotype (FOXP3, CD25 expression)?

I am planning to run some preliminary experiments to assess the expression of a Treg-like phenotype by checking markers such as FOXP3 and CD25. Could anyone recommend a suitable cell line for this...

20 March 2025 3,022 3 View

Is it necessary for researchers to assess the expression of actin each time they perform qRT-PCR for different genes on the same set of samples cDNA?

I AM DOING expression analysis of multiple genes from same cDNA sample and since there are many genes I will perform qPCR over few days9 in a weekend), do i have to prepare actin for each time i...

01 February 2024 6,213 1 View

Need some help regarding 3d Model predicted by i-tasser?

I made a 3d model for IRS2 protein using i tasser software and then refined the predicted model on mod refiner server , But the ramachandran plot score on SAVES serves is not coming in acceptable...

09 July 2023 8,653 1 View

I've got phenol contamination in my dissolved RNA sample in DPEC water. Is there anyway to remove this contamination?

I extracted RNA from Uterine fibroid tissue by using TRIzol Method.

16 June 2023 3,312 2 View

How can we compare two different samples having totally different sample size?

On the Ualcan website, they have compared the normal sample with the tumor sample, both of which have a different sample size, and based on that they have calculated the statistical significance...

01 January 1970 6,746 2 View