Hi everyone,
Recently I am working on oligonucleotide. I have a fluorophore tagged DNA for my experiment. My DNA is tagged with 6-FAM at 3' end. It is suggested that FAM tagged DNA should remain in solutions with 7.2 pH or higher to limit degradation. There are TE buffer or PBS which can be used to dissolve it. For my experiment I want to avoid EDTA and salt actually. But to maintain higher pH ,I have to go with a buffer solution. Now my question is:
Can I use phosphate buffer of pH 8 instead of TE buffer or PBS to dissolve this FAM tagged DNA?
If any other buffer solution will be useful, then please suggest me.