Could anyone help me to find the best composition for a buffer lysis solution to extract a pure DNA from a microbial community of bacteria and yeast in a cellulosic matrix (a cellulosic pellicule of a kombucha)? to get cells the first step is a mechanical treatment, it would be better to get soft lysis in the next step to ensure the integrity and the purity of the DNA. Otherwise, cellulase treatment was not efficient to get an important amount of DNA, does anyone work on a complex community DNA extraction?

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