Which buffer is good for buffering capacity and interaction with protein?
Both are working,
the choice depends more on your protein itself than on the column
I have to stop my enzyme reaction pH(8.5) by perchloric acid and further neutralized by KHCO3. How to determine the amount of acid that is required to stop the reaction and is there any...
05 June 2017 913 5 View
Iam measuring the absorption of substrate in a enzymatic reaction to find out the kinetic parameters Km and Vmax. Plot of the curve (absorbance vs time) obtained in a exponential decay manner. To...
11 December 2016 8,970 3 View
Iam trying to prepare 0.1M ZnCl2 solution but it is forming white precipitate. What are factors that influence its solubility in water or what might be the probable solvent to make it soluble.
04 May 2015 4,970 8 View
I have read in one of the article that they have crystallized protein by screening the protein solution which was partially aggregated before even setting and the drop and finally they got good...
03 April 2015 5,975 8 View
I have purified a protein of 36kDa with Ni-NTA affinity chrmotography and initially protein was eluted with 100-500mM imidazole. When elution was started with 300mM imidazole and so on protein...
10 November 2014 9,068 13 View
If I have expressed a functional protein in e.coli,how the activity of the protein in bacterial cytoplasm will effect its survival? If it is a metabolic enzyme and by chance it is involved in...
04 May 2014 9,775 2 View
I have solubilized my protein with 0.3% sarcosine and purified by using Ni-NTA,during purification most protein is going into flow through. I have diluted my sonicated sample to 0.1% sarcosine...
04 May 2014 8,290 5 View
I have sonicated my bacterial cell pellet with 0.5% sarkosyl and have a sufficient amount in soluble fraction. In another condition, I have used 50mM each of L-Arginine and glycyl glycine in...
03 April 2014 3,051 3 View
My protein has 59 kDa and I got gst along with it during purification. I am confused whether my protein is degrading or my induced colony has both native and/or vector with insert.
11 December 2013 8,612 1 View
I have read some articles, in which they have used BL21 and in other some cases it is M15 cells. So, which is the better host strain for expression and why ?
08 September 2013 5,642 9 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?
31 July 2024 3,479 5 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View