How to determine the amount of acid required to stop the enzyme reaction?

I have to stop my enzyme reaction pH(8.5) by perchloric acid and further neutralized by KHCO3. How to determine the amount of acid that is required to stop the reaction and is there any...

05 June 2017 746 5 View

How to calculate the slope of a line that resembles the exponential decay?

Iam measuring the absorption of substrate in a enzymatic reaction to find out the kinetic parameters Km and Vmax. Plot of the curve (absorbance vs time) obtained in a exponential decay manner. To...

11 December 2016 8,770 3 View

Is it possible to crystallize proteins which precipitate immediately after purification?

I have read in one of the article that they have crystallized protein by screening the protein solution which was partially aggregated before even setting and the drop and finally they got good...

03 April 2015 5,861 8 View

How might one overcome protein precipitation during elution ?

10 November 2014 8,925 13 View

Anyone familiar with the fate of expressed protein in E.coli?

If I have expressed a functional protein in e.coli,how the activity of the protein in bacterial cytoplasm will effect its survival? If it is a metabolic enzyme and by chance it is involved in...

04 May 2014 9,651 2 View

How can I eliminate the interference of sarcosine in the binding of protein to ni-nta?

I have solubilized my protein with 0.3% sarcosine and purified by using Ni-NTA,during purification most protein is going into flow through. I have diluted my sonicated sample to 0.1% sarcosine...

04 May 2014 8,160 5 View

What is the minimum percentage of sarkosyl that is allowed for a lysis buffer when extracting native proteins from inclusion bodies?

I have sonicated my bacterial cell pellet with 0.5% sarkosyl and have a sufficient amount in soluble fraction. In another condition, I have used 50mM each of L-Arginine and glycyl glycine in...

03 April 2014 2,923 3 View

I have purified my protein coupled to gst tag and i got several non specific bands during purification, how to reduce non-specificity ?

My protein has 59 kDa and I got gst along with it during purification. I am confused whether my protein is degrading or my induced colony has both native and/or vector with insert.

11 December 2013 8,488 1 View

Is it possible to use E.coli BL21(DE3) as a host for the expression of my target protein cloned in pQE 30 vector?

I have read some articles, in which they have used BL21 and in other some cases it is M15 cells. So, which is the better host strain for expression and why ?

08 September 2013 5,430 9 View

Could someone suggest some methods for the purification of proteins from inclusion bodies?

As most of the proteins are going into the insoluble fraction as an aggregate and a very low amount in soluble fraction...

08 September 2013 579 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to calculate stress at the cross section of spherical particles within a matrix?

Hi all, I am currently working on some background calculations before I start a new project related to metal matrix particulate composites. I am trying to find a way to calculate the stress at...

19 February 2021 8,503 3 View