What is the mass% and mole% of water in the 11ml solution?

10 January 2021 127 2 View

CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Can you see bands from DNA on SDS-PAGE?

If a protein sample is contaminated with nucleic acid, will these DNA/RNA run on the gel and show bands? (gradient gel, 4-20%, MW-marker: 14.4-116 kDa)

05 December 2020 9,484 3 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View

Direct Oblimin rotation and negative factor loadings?

Dear researchers, I hope you can assist me or direct me to some literature with answers. I have conducted an EFA on 22 variables which revealed 4 factors. I have used the results of the EFA to...

15 November 2020 8,078 10 View

Is well-being related to higher academic achievements?

Hello fellow researchers, I am writing an introduction on a new article on social marginalization in school, where I wish to refer to leading research on well-being (or school belonging) and...

13 May 2020 4,551 4 View

How to resolve CPCe run-time error "9"?

Hi When trying to export the analysis I have done of my images from CPCe I get the following error message which says "Run-time error "9": Subscript out of range". I have uploaded a screenshot...

30 April 2020 1,972 1 View

Best software to draw SEM figure?

Hi all, I am looking for the best software package to make professional looking figures when using structural equation modelling. So far I have used Amos Graphics (v. 26), but in most papers and...

24 April 2020 5,543 8 View

The role of available P vs total P: Do they affect plant species composition and biodiversity in a similar or different way?

The nutrient P can be measured either as available P or total P. My question is: When looking at the change in plant species compositions, does it make a difference whether it is modeled against...

01 November 2018 9,130 2 View

Does anyone want (for the cost of postage if mailed) my nearly complete set of issues of "Frontiers in Ecology & the Environment" 2003-2015?

Journals no longer needed.

30 June 2018 554 40 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Am i able to conduct a factor analysis on each of my subscales, as opposed to the whole scale?

My psychometric scale contains 38 items in total, with 4 subscales (20, 6, 6 & 6 items respectively? Can i run a factor analysis for each subscale rather than the scale as a whole for the...

24 February 2021 8,856 8 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

How many indicators do I need to represent a latent construct?

Hi folks! We did a large survey and planned to use 3 indicators to represent a latent construct (Sense of Place/SoP) in a latent regression model (structural equation modeling). However, one of...

15 February 2021 7,705 3 View

How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View